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1. Agrobacterium T-DNA integration in somatic cells does not require the activity of DNA polymerase theta SCOPUS Scopus数据库 SCIE Web of Science核心 NSTL国家科技图书文献中心

Nishizawa-Yokoi, Aya... | Saika, Hiroaki ... - 《The New Phytologist》 - 2021,229(5) - 2859～2872 - 共14页 - 被引量：21

摘要：Integration of Agrobacterium tumefaciens transferred DNA (T-DNA) into the plant genome is the last step required for stable plant genetic transformation. The mechanism of T-DNA integration remains con...

关键词： Agrobacterium& | 8208 | mediated plant transformation | Arabidopsis thaliana | DNA polymerase & | 952 | Oryza sativa (rice) | T& | DNA integration | DNA junction sequences

2. Agrobacterium-delivered VirE2 interacts with host nucleoporin CG1 to facilitate the nuclear import of VirE2-coated T complex NSTL国家科技图书文献中心

Li, Xiaoyang | Yang, Qinghua ... - 《Proceedings of the National Academy of Sciences of the United States of America.》 - 2020,117(42) - 26389～26397 - 共9页 - 被引量：10

摘要：Agrobacterium tumefaciens is the causal agent of crown gall disease. The bacterium is capable of transferring a segment of single-stranded DNA (ssDNA) into recipient cells during the transformation pr...

关键词： Agrobacterium | VirE2 | T-complex | CG1 | nuclear import | SINGLE-STRANDED-DNA | TUMEFACIENS-MEDIATED TRANSFORMATION | VIRULENCE PROTEIN VIRE2 | LOCALIZATION SIGNAL | BINDING-PROTEIN...

3. Application of Cas12a and nCas9-activation-induced cytidine deaminase for genome editing and as a non-sexual strategy to generate homozygous/multiplex edited plants in the allotetraploid genome of tobacco SCOPUS Scopus数据库 SCIE Web of Science核心 NSTL国家科技图书文献中心

Hsu, Chen-Tran | Cheng, Yu-Jung ... - 《Plant Molecular Biology》 - 2019,101(4/5) - 355～371 - 共17页 - 被引量：13

摘要：Key message Protoplasts can be used for genome editing using several different CRISPR systems, either separately or simultaneously, and that the resulting mutations can be recovered in regenerated non...

关键词： CRISPR | Cas | Chimeric plants | Cas12a | Cytidine deaminase | Polyploid genome editing

4. An armadillo-domain protein participates in a telomerase interaction network SCOPUS Scopus数据库 SCIE Web of Science核心 NSTL国家科技图书文献中心

Dokladal, Ladislav | Benkova, Eva ... - 《Plant Molecular Biology》 - 2018,97(4/5) - 407～420 - 共14页 - 被引量：6

摘要：Arabidopsis and human ARM protein interact with telomerase. Deregulated mRNA levels of DNA repair and ribosomal protein genes in an Arabidopsis arm mutant suggest non-telomeric ARM function. The human...

关键词： Armadillo/beta-catenin-like repeat | ARMC6 | AtTERT | Homologous recombination | Protein-protein interaction | Telomerase activity

5. Somaclonal variation does not preclude the use of rice transformants for genetic screening SCIE Web of Science核心 SCOPUS Scopus数据库 NSTL国家科技图书文献中心

Wei, Fu-Jin | Kuang, Lin-Yun ... - 《The Plant Journal》 - 2016,85(5) - 648～659 - 共12页 - 被引量：19

摘要：Rice (Oryza sativa) is one of the world's most important crops. Rice researchers make extensive use of insertional mutants for the study of gene function. Approximately half a million flanking sequenc...

关键词： Agrobacterium-mediated transformation | indels | regeneration | rice | single-nucleotide polymorphisms | somaclonal variation

6. Agrobacterium T-DNA integration into the plant genome can occur without the activity of key non-homologous end-joining proteins SCOPUS Scopus数据库 SCIE Web of Science核心 NSTL国家科技图书文献中心

Park, So-Yon | Vaghchhipawala, Zari...... - 《The Plant Journal》 - 2015,81(6) - 934～946 - 共13页 - 被引量：27

摘要：Non-homologous end joining (NHEJ) is the major model proposed for Agrobacterium T-DNA integration into the plant genome. In animal cells, several proteins, including KU70, KU80, ARTEMIS, DNA-PKcs, DNA...

关键词： Agrobacterium | non-homologous end joining | T-DNA integration | plant transformation | Arabidopsis thaliana | Nicotiana benthamiana

7. Is VIP1 important for Agrobacterium-mediated transformation? SCOPUS Scopus数据库 SCIE Web of Science核心 NSTL国家科技图书文献中心

Shi, Yong | Lee, Lan-Ying ... - 《The Plant Journal》 - 2014,79(5) - 848～860 - 共13页 - 被引量：37

摘要：Agrobacterium genetically transforms plants by transferring and integrating T-(transferred) DNA into the host genome. This process requires both Agrobacterium and host proteins. VirE2 interacting prot...

关键词： Agrobacterium | plant transformation | VirE2 interacting protein 1 | VirE2 | Arabidopsis thaliana | Nicotiana benthamiana | protein subcellular localization

8. Overexpression of Several Arabidopsis Histone Genes Increases Agrobacterium-Mediated Transformation and Transgene Expression in Plants. SCIE Web of Science核心 NSTL国家科技图书文献中心

Tenea, Gabriela N. | Spantzel, Joerg ... - 《The Plant Cell》 - 2009,21(10) - 3350～3367 - 共18页

摘要：The Arabidopsis thaliana histone H2A-1 is important for Agrobacterium tumefaciens-mediated plant transformation. Mutation of HTA1, the gene encoding histone H2A-1, results in decreased T-DNA integrati...

9. Characterization of T-Circles and Their Formation Reveal Similarities to Agrobacterium T-DNA Integration Patterns

Singer, Kamy | Lee, Lan-Ying ... - 《frontiers in plant science》 - 2022,13 - 共15页 - 被引量：3

摘要：Agrobacterium transfers T-DNA to plants where it may integrate into the genome. Non-homologous end-joining (NHEJ) has been invoked as the mechanism of T-DNA integration, but the role of various NHEJ p...

关键词： Agrobacterium | Arabidopsis thaliana | Ku80 | Nicotiana benthamiana | T-circles | T-DNA integration | VirD2 | STRANDED TRANSFERRED DNA | VIRD2 PROTEIN | CHROMOSOMAL TRANSLOCATIONS...

10. Agrobacterium VirE2 Protein Modulates Plant Gene Expression and Mediates Transformation From Its Location Outside the Nucleus

Lapham, Rachelle A. | Lee, Lan-Ying ... - 《frontiers in plant science》 - 2021,12 - 共20页 - 被引量：5

摘要：Agrobacterium effector protein VirE2 is important for plant transformation. VirE2 likely coats transferred DNA (T-DNA) in the plant cell and protects it from degradation. VirE2 localizes to the plant ...

关键词： Agrobacterium | Arabidopsis | plant transformation | VirE2 | protein subcellular localization | transcriptome | proteome | SINGLE-STRANDED-DNA | T-DNA | TRANSFERRED DNA...