Portela, Rui M. C. |  Vogl, Thomas... -  《ACS Synthetic Biology》 - 2017,6(3) - 471～484 - 共14页 - 被引量：58

摘要：Synthetic biology and metabolic engineering experiments frequently require the fine-tuning of gene expression to balance and optimize protein levels of regulators or metabolic enzymes. A key concept o...

关键词： core promoter |  promoter library |  transcriptional fine-tuning |  synthetic biology |  yeast |  computational design

Fischer, Jasmin E. |  Glieder, Anton -  《Current opinion in biotechnology》 - 2019,59 - 175～181 - 共7页 - 被引量：43

摘要：In the past two to five years innovative DNA tools and inventive methodologies accelerated the speed of engineering of Komagataella phaffii (Pichia pastoris) for the efficient expression of intracellu...

Schusterbauer, Veron... |  Fischer, Jasmin E.... -  《journal of fungi》 - 2022,8(10) - 共23页

摘要：The industrially important non-conventional yeast Komagataella phaffii suffers from low rates of homologous recombination, making site specific genetic engineering tedious. Therefore, genome editing u...

关键词： CRISPR |  Cas technology |  non-conventional yeasts |  genome analysis |  non-homologous end joining |  PICHIA-PASTORIS |  GUIDE RNA |  EXPRESSION |  GENE |  PATHWAY...